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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MUC13 Polyclonal antibody

MUC13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29832-1-AP

Synonyms

DRCC1, FLJ20063, MUC 13, MUC13, Mucin 13, RECC

Validation Data Gallery

Applications:
  • WB analysis using 29832-1-AP

    WB analysis using 29832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29832-1-AP (MUC13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29832-1-AP

    IHC staining of human colon cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29832-1-AP

    IHC staining of human colon cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 29832-1-AP

    IHC staining of human colon using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 29832-1-AP

    IHC staining of human colon using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 29832-1-AP

    IHC staining of human stomach cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 29832-1-AP

    IHC staining of human stomach cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHCT 116 cells, HEK-293 cells, HT-29 cells, SW480 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

29832-1-AP targets MUC13 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC13 fusion protein Ag31090 相同性解析による交差性が予測される生物種
Full Name mucin 13, cell surface associated
Calculated molecular weight 55 kDa
Observed molecular weight70-120 kDa
GenBank accession numberNM_033049
Gene symbol MUC13
Gene ID (NCBI) 56667
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins which is normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).

Protocols

Product Specific Protocols
WB protocol for MUC13 antibody 29832-1-APDownload protocol
IHC protocol for MUC13 antibody 29832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols