Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
13995-1-AP targets MTSS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5080 Product name: Recombinant human MTSS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-473 aa of BC023998 Sequence: SQDAFQSKSPSPMPPEAPNQRRKEKREPDPNGGGPTTASGPPAAAEEAQRPRSMTVSAATRPGEEMEACEELALALSRGLQLDTQRSSRDSLQCSSGYSTQTTTPCCSEDTIPSQVSDYDYFSVSGDQEADQQEFDKSSTIPRNSDISQSYRRMFQAKRPASTAGLPTTLGPAMVTPGVATIRRTPSTKPSVRRGTIGAGPIPIKTPVIPVKTPTVPDLPGVLPAPPDGPEERGEHSPESPSVGEGPQGVTSMPSSMWSGQASVNPPLPGPKPSIPEEHRQAIPESEAEDQEREPPSATVSPGQIPESDPADLSPRDTPQGEDMLNAIRRGVKLKKTTTNDRSAPRFS 相同性解析による交差性が予測される生物種 |
| Full Name | metastasis suppressor 1 |
| Calculated molecular weight | 82 kDa |
| Observed molecular weight | 82 kDa |
| GenBank accession number | BC023998 |
| Gene Symbol | MTSS1 |
| Gene ID (NCBI) | 9788 |
| RRID | AB_3085428 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43312 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTSS1 (metastasis suppressor 1), also known as MIM (missing-in-metastasis), MIM-B, BEG4 (Basal cell carcinoma-enriched gene 4) or KIAA0429, is one of the defining members of the I-BAR family of negative membrane curvature-sensing proteins first identified as being deleted in metastatic bladder cancer. MTSS1 over-expression is associated with enhanced cell migration, resulting in tumorigenesis, invasion and metastasis (PMID: 27230279). It is reported that the expression of MTSS1 is associated with poor prognosis in various cancers,such as bladder, gastric, breast and so on (PMID: 32052679).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTSS1 antibody 13995-1-AP | Download protocol |
| IHC protocol for MTSS1 antibody 13995-1-AP | Download protocol |
| WB protocol for MTSS1 antibody 13995-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cytoskeleton (Hoboken) Metastasis suppressor 1 interacts with α-actinin 4 to affect its localization and regulate formation of membrane ruffling.
| ||
ACS Nano Nonswellable Hydrogel Patch with Tissue-Mimetic Mechanical Characteristics Remodeling In Vivo Microenvironment for Effective Adhesion Prevention |
