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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTPN Polyclonal antibody (13508-1-AP)

    WB analysis using 13508-1-AP (same clone as 13508-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13508-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MTPN Polyclonal antibody (13508-1-AP)

    WB analysis of human brain using 13508-1-AP (same clone as 13508-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13508-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MTPN Polyclonal antibody (13508-1-AP)

    IHC staining of human colon using 13508-1-AP (same clone as 13508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13508-1-AP (MTPN antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13508-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MTPN Polyclonal antibody (13508-1-AP)

    IHC staining of human heart using 13508-1-AP (same clone as 13508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 13508-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MTPN Polyclonal antibody (13508-1-AP)

    IHC staining of human heart using 13508-1-AP (same clone as 13508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 13508-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTPN Polyclonal antibody (13508-1-AP)

    IF Staining of HepG2 using 13508-1-AP (same clone as 13508-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTPN antibody (13508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13508-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTPN Polyclonal antibody (13508-1-AP)

    IF Staining of HeLa using 13508-1-AP (same clone as 13508-1-PBS)

    Immunofluorescent analysis of Hela cells, using MTPN antibody 13508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13508-1-PBS in a different storage buffer formulation.

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MTPN Polyclonal antibody, PBS Only

MTPN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13508-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13508-1-PBS targets MTPN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTPN fusion protein Ag4419 相同性解析による交差性が予測される生物種
Full Name myotrophin
Calculated molecular weight 118 aa, 13 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC028093
Gene Symbol MTPN
Gene ID (NCBI) 136319
RRIDAB_2148129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58546
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Myotrophin (MTPN) is a ubiquitously expressed 12 kDa cytoplamic protein that was first isolated from the hearts of spontaneously hypertensive rats. Protein sequence analysis revealed that MTPN was composed of 118 amino acids and was occupied by two and a half internal 33 amino acid ankyrin repeats. MTPN stimulates protein synthesis and increases the expression of a number of cardiac marker genes (such as atrial natriuretic factor and b-myosin heavy chain) in cardiomyocytes leading to hypertrophy. In skeletal muscle, MTPN is also a positive growth factor in promoting skeletal muscle growth.