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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTMR9 Recombinant antibody (83694-4-RR)

    WB analysis using 83694-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83694-4-RR (MTMR9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MTMR9 Recombinant antibody (83694-4-RR)

    IF Staining of HeLa using 83694-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR9 antibody (83694-4-RR, Clone: 240167G7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A549 cells using MTMR9 Recombinant antibody (83694-4-RR)

    FC experiment of A549 using 83694-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HeLa cells using MTMR9 Recombinant antibody (83694-4-RR)

    FC experiment of HeLa using 83694-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83694-4-RR

    Affinity and Kinetic Characterization of 83694-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83694-4-RR against Human MTMR9 were performed. The affinity constant is below 1 pM.

MTMR9 Recombinant monoclonal antibody

MTMR9 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83694-4-RR
Clone No.240167G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240167G7, C8orf9, Inactive phosphatidylinositol 3-phosphatase 9, LIP STYX, MTMR8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, U2OS cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83694-4-RR targets MTMR9 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34134

Product name: Recombinant human MTMR9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 424-549 aa of BC022003

Sequence: MLFEHAYASQFGTFLGNNESERCKLKLQQKTMSLWSWVNQPSELSKFTNPLFEANNLVIWPSVAPQSLPLWEGIFLRWNRSSKYLDEAYEEMVNIIEYNKELQAKVNILRRQLAELETEDGMQESP

相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 9
Calculated molecular weight 63 kDa
Observed molecular weight~60 kDa
GenBank accession numberBC022003
Gene Symbol MTMR9
Gene ID (NCBI) 66036
RRIDAB_3671297
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96QG7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).

Protocols

Product Specific Protocols
IF protocol for MTMR9 antibody 83694-4-RRDownload protocol
WB protocol for MTMR9 antibody 83694-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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