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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of HeLa using 12299-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of A375 using 12299-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of HeLa using 12299-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MTMR8 Polyclonal antibody (12299-1-AP)

    WB analysis of human placenta using 12299-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12299-1-AP (MTMR8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MTMR8 Polyclonal antibody (12299-1-AP)

    IF Staining of A375 using 12299-1-AP

    Immunofluorescent analysis of A375 cells using 12299-1-AP (MTMR8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MTMR8 Polyclonal antibody

MTMR8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12299-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Phosphatidylinositol-3-phosphate phosphatase, Phosphatidylinositol-3,5-bisphosphate 3-phosphatase, Myotubularin-related protein 8, myotubularin related protein 8, EC:3.1.3.95

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A375 cells, human placenta tissue
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12299-1-AP targets MTMR8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTMR8 fusion protein Ag2944 相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 8
Calculated molecular weight 704 aa, 79 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC012399
Gene Symbol MTMR8
Gene ID (NCBI) 55613
RRIDAB_2298075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MTMR8 antibody 12299-1-APDownload protocol
IF protocol for MTMR8 antibody 12299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols