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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MTMR2 Monoclonal antibody (67312-1-Ig)

    WB analysis of HeLa using 67312-1-Ig (same clone as 67312-1-PBS)

    WB result of MTMR2 antibody (67312-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR2 transfected HeLa cells. This data was developed using the same antibody clone with 67312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MTMR2 Monoclonal antibody (67312-1-Ig)

    WB analysis of HeLa using 67312-1-Ig (same clone as 67312-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67312-1-Ig (MTMR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MTMR2 Monoclonal antibody (67312-1-Ig)

    WB analysis of HEK-293 using 67312-1-Ig (same clone as 67312-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67312-1-Ig (MTMR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MTMR2 Monoclonal antibody (67312-1-Ig)

    WB analysis of Jurkat using 67312-1-Ig (same clone as 67312-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67312-1-Ig (MTMR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67312-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using MTMR2 Monoclonal antibody (67312-1-Ig)

    WB analysis of pig brain using 67312-1-Ig (same clone as 67312-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67312-1-Ig (MTMR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67312-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MTMR2 Monoclonal antibody, PBS Only

MTMR2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67312-1-PBS
Clone No.1B11B7

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB, Indirect ELISA

Synonyms

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67312-1-PBS targets MTMR2 in WB, Indirect ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28413

Product name: Recombinant human MTMR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 294-643 aa of BC052990

Sequence: KRSKEDEKYLQAIMDSNAQSHKIFIFDARPSVNAVANKAKGGGYESEDAYQNAELVFLDIHNIHVMRESLRKLKEIVYPNIEETHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDGWDRTAQLTSLAMLMLDGYYRTIRGFEVLVEKEWLSFGHRFQLRVGHGDKNHADADRSPVFLQFIDCVWQMTRQFPTAFEFNEYFLITILDHLYSCLFGTFLCNSEQQRGKENLPKRTVSLWSYINSQLEDFTNPLYGSYSNHVLYPVASMRHLELWVGYYIRWNPRMKPQEPIHNRYKELLAKRAELQKKVEELQREISNRSTSSSERASSPAQCVTPVQTVV

相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 2
Calculated molecular weight 73 kDa
Observed molecular weight 66-73 kDa
GenBank accession numberBC052990
Gene Symbol MTMR2
Gene ID (NCBI) 8898
RRIDAB_2882572
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13614
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

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