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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MTMR2 Polyclonal antibody (14372-1-AP)

    WB analysis of HeLa using 14372-1-AP

    WB result of MTMR2 antibody (14372-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using MTMR2 Polyclonal antibody (14372-1-AP)

    WB analysis using 14372-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human cerebellum tissue using MTMR2 Polyclonal antibody (14372-1-AP)

    WB analysis of human cerebellum using 14372-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human cerebellum tissue using MTMR2 Polyclonal antibody (14372-1-AP)

    WB analysis of human cerebellum using 14372-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse cerebellum tissue using MTMR2 Polyclonal antibody (14372-1-AP)

    IP experiment of mouse cerebellum using 14372-1-AP

    IP result of anti-MTMR2 (IP:14372-1-AP, 4ug; Detection:14372-1-AP 1:300) with mouse cerebellum tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using MTMR2 Polyclonal antibody (14372-1-AP)

    IHC staining of human endometrial cancer using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using MTMR2 Polyclonal antibody (14372-1-AP)

    IHC staining of human endometrial cancer using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using MTMR2 Polyclonal antibody (14372-1-AP)

    IHC staining of human cerebellum using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using MTMR2 Polyclonal antibody (14372-1-AP)

    IHC staining of human cerebellum using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

MTMR2 Polyclonal antibody

MTMR2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14372-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human cerebellum tissue, NIH/3T3 cells
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inhuman endometrial cancer tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14372-1-AP targets MTMR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTMR2 fusion protein Ag5745 相同性解析による交差性が予測される生物種
Full Name myotubularin related protein 2
Calculated molecular weight 73 kDa
Observed molecular weight 66-73 kDa
GenBank accession numberBC052990
Gene Symbol MTMR2
Gene ID (NCBI) 8898
RRIDAB_2282336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13614
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

Protocols

Product Specific Protocols
WB protocol for MTMR2 antibody 14372-1-APDownload protocol
IHC protocol for MTMR2 antibody 14372-1-APDownload protocol
IP protocol for MTMR2 antibody 14372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols