Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55325-1-AP targets MTIF2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial translational initiation factor 2 |
| Calculated molecular weight | 81 kDa |
| Observed molecular weight | 81 kDa |
| GenBank accession number | NM_002453 |
| Gene Symbol | MTIF2 |
| Gene ID (NCBI) | 4528 |
| RRID | AB_3670248 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46199 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTIF2 belongs to the IF-2 family. It is one of the essential components for the initiation of protein synthesis. MTIF2 protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. It is also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. This antibody is specific to MTIF2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTIF2 antibody 55325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |