Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HaCaT cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32122-1-AP targets MTHFSD in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MTHFSD fusion protein Ag37100 相同性解析による交差性が予測される生物種 |
| Full Name | methenyltetrahydrofolate synthetase domain containing |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC112059 |
| Gene Symbol | MTHFSD |
| Gene ID (NCBI) | 64779 |
| RRID | AB_3670199 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q2M296 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTHFSD is an RNA-binding protein that contains a methyltetrahydrofolate synthase structural domain and an RRM structural domain. In healthy motor neurons, MTHFSD is predominantly located in the nucleus, and studies have shown that MTHFSD, as a novel marker of stress granules, may be associated with aggregated proteins involved in amyotrophic lateral sclerosis (ALS).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTHFSD antibody 32122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |