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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTHFS Polyclonal antibody (13114-1-AP)

    WB analysis using 13114-1-AP (same clone as 13114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MTHFS Polyclonal antibody (13114-1-AP)

    WB analysis of HeLa using 13114-1-AP (same clone as 13114-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MTHFS Polyclonal antibody (13114-1-AP)

    WB analysis of Jurkat using 13114-1-AP (same clone as 13114-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using MTHFS Polyclonal antibody (13114-1-AP)

    WB analysis of human stomach using 13114-1-AP (same clone as 13114-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using MTHFS Polyclonal antibody (13114-1-AP)

    WB analysis of human liver using 13114-1-AP (same clone as 13114-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using MTHFS Polyclonal antibody (13114-1-AP)

    WB analysis of U-937 using 13114-1-AP (same clone as 13114-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MTHFS Polyclonal antibody (13114-1-AP)

    IP experiment of HeLa using 13114-1-AP (same clone as 13114-1-PBS)

    IP result of anti-MTHFS (IP:13114-1-AP, 4ug; Detection:13114-1-AP 1:2000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFS Polyclonal antibody (13114-1-AP)

    IHC staining of human liver cancer using 13114-1-AP (same clone as 13114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFS Polyclonal antibody (13114-1-AP)

    IHC staining of human liver cancer using 13114-1-AP (same clone as 13114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFS Polyclonal antibody (13114-1-AP)

    IHC staining of human liver cancer using 13114-1-AP (same clone as 13114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MTHFS Polyclonal antibody (13114-1-AP)

    IHC staining of human liver cancer using 13114-1-AP (same clone as 13114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13114-1-PBS in a different storage buffer formulation.

MTHFS Polyclonal antibody, PBS Only

MTHFS Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13114-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Methenyl-THF synthetase, Methenyl THF synthetase, HsT19268, EC:6.3.3.2, 5-formyltetrahydrofolate cyclo-ligase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13114-1-PBS targets MTHFS in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3781

Product name: Recombinant human MTHFS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-203 aa of BC019921

Sequence: MAAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQDEIETEEIIKDIFQRGKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEVKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSSTA

相同性解析による交差性が予測される生物種
Full Name 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
Calculated molecular weight 203 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC019921
Gene Symbol MTHFS
Gene ID (NCBI) 10588
RRIDAB_2250977
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49914
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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