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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTFR1 Polyclonal antibody

MTFR1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17778-1-AP

Synonyms

CHPPR, FAM54A2, KIAA0009, MTFR1

Validation Data Gallery

Applications:
  • WB analysis using 17778-1-AP

    WB analysis using 17778-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17778-1-AP (MTFR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 17778-1-AP

    WB analysis of A549 using 17778-1-AP

    WB result of MTFR1 antibody (17778-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTFR1 transfected A549 cells.

  • IP experiment of mouse testis using 17778-1-AP

    IP experiment of mouse testis using 17778-1-AP

    IP result of anti-MTFR1 (IP:17778-1-AP, 4ug; Detection:17778-1-AP 1:800) with mouse testis tissue lysate 4800ug.

Tested Applications

Positive WB detected inA549 cells, HCT 116 cells, HepG2 cells, SW480 cells
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

17778-1-AP targets MTFR1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTFR1 fusion protein Ag10857 相同性解析による交差性が予測される生物種
Full Name mitochondrial fission regulator 1
Calculated molecular weight 333 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC036116
Gene symbol MTFR1
Gene ID (NCBI) 9650
RRIDAB_2282301
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTFR1, also known as CHPPR, is a mitochondria protein. It may play a role in mitochondrial aerobic respiration. It may also regulate mitochondrial organization and fission.

Protocols

Product Specific Protocols
WB protocol for MTFR1 antibody 17778-1-APDownload protocol
IP protocol for MTFR1 antibody 17778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols