Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, HeLa cells, HepG2 cells, LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31892-1-AP targets MTERF4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32334 Product name: Recombinant human MTERFD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 43-381 aa of NM_182501 Sequence: LTTASNGGVIEELSCVRSNNYVQEPECRRNLVQCLLEKQGTPVVQGSLELERVMSSLLDMGFSNAHINELLSVRRGASLQQLLDIISEFILLGLNPEPVCVVLKKSPQLLKLPIMQMRKRSSYLQKLGLGEGKLKRVLYCCPEIFTMRQQDINDTVRLLKEKCLFTVQQVTKILHSCPSVLREDLGQLEYKFQYAYFRMGIKHPDIVKSEYLQYSLTKIKQRHIYLERLGRYQTPDKKGQTQIPNPLLKDILRVSEAEFLARTACTSVEEFQVFKKLLAREEEESESSTSDDKRASLDEDEDDDDEEDNDEDDNDEDDDDEDDDEAEDNDEDEDDDEEE* 相同性解析による交差性が予測される生物種 |
| Full Name | MTERF domain containing 2 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | NM_182501 |
| Gene Symbol | MTERFD2 |
| Gene ID (NCBI) | 130916 |
| RRID | AB_3670135 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q7Z6M4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The MTERF protein family plays a key role in controlling mitochondrial DNA gene expression in mammals. Mitochondrial transcription termination factor 4 (MTERF4, also known as MTERFD2) regulates mitochondrial ribosome biogenesis and translation (PMID: 21531335). It can form a stoichiometric complex with the ribosomal RNA methyl-transferase NSUN4 and is necessary for the recruitment of this factor to the large ribosomal subunit. Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation (PMID: 21531335).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTERF4 antibody 31892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |