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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTERFD2 Polyclonal antibody (31892-1-AP)

    WB analysis using 31892-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31892-1-AP (MTERFD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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MTERF4 Polyclonal antibody

MTERF4 Polyclonal Antibody for WB, ELISA
Cat No. 31892-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MTERFD2, Transcription termination factor 4, mitochondrial, mTERF domain-containing protein 2, MTERF Domain Containing 2 , Mitochondrial transcription termination factor 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31892-1-AP targets MTERF4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTERF4 fusion protein Ag32334 相同性解析による交差性が予測される生物種
Full Name MTERF domain containing 2
Calculated molecular weight44 kDa
Observed molecular weight37 kDa
GenBank accession numberNM_182501
Gene Symbol MTERFD2
Gene ID (NCBI) 130916
RRIDAB_3670135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ7Z6M4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MTERF protein family plays a key role in controlling mitochondrial DNA gene expression in mammals. Mitochondrial transcription termination factor 4 (MTERF4, also known as MTERFD2) regulates mitochondrial ribosome biogenesis and translation (PMID: 21531335). It can form a stoichiometric complex with the ribosomal RNA methyl-transferase NSUN4 and is necessary for the recruitment of this factor to the large ribosomal subunit. Loss of MTERF4 leads to defective ribosomal assembly and a drastic reduction in translation (PMID: 21531335).

Protocols

Product Specific Protocols
WB protocol for MTERF4 antibody 31892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols