Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, MDCK cells |
| Positive IP detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25780-1-AP targets MTCL1 in WB, IP, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22718 Product name: Recombinant human KIAA0802 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 775-976 aa of BC040542 Sequence: IDHSPVVQDPFQKGLRAGSRSRSAEPRPELGPGQETGTNSRGRSPSPIGVGSEMCREEGGEGTPVKQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAAHGPPGLHSDSHSLGDTAEPGPMEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHSPTASQPQSPGDPTSLEEHGGEEPPEEQPHRDASLHGLSQYNSL 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0802 |
| Calculated molecular weight | 1905 aa, 209 kDa |
| Observed molecular weight | 250 kDa |
| GenBank accession number | BC040542 |
| Gene Symbol | MTCL1 |
| Gene ID (NCBI) | 23255 |
| RRID | AB_3669499 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4B5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Microtubule cross‐linking factor 1 (MTCL1) is a MT‐regulating protein that plays essential roles in accumulating the non-centrosomal MTs into the apicobasal MT bundles. MTCL1, identified as a binding protein of the cell polarity‐regulating kinase, PAR‐1b/MARK2, is recruited to the Golgi membranes through interactions with CLASPs and AKAP450/CG-NAP, and promotes microtubule growth from the Golgi membrane. Western blot analysis detected MTCL1 at an apparent molecular mass of 250 kDa as indicated in the literature (PMID: 23902687, 28283581).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTCL1 antibody 25780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |