Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat testis tissue |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 20 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
| CoIP | See 2 publications below |
Product Information
16888-1-AP targets MTCH2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MTCH2 fusion protein Ag10399 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial carrier homolog 2 (C. elegans) |
| Calculated molecular weight | 303 aa, 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC000875 |
| Gene Symbol | MTCH2 |
| Gene ID (NCBI) | 23788 |
| RRID | AB_2266733 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6C9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| IHC protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| IF protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| IP protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology
| ||
Sci Adv ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution | ||
Cell Death Dis MTCH2 regulates NRF2-mediated RRM1 expression to promote melanoma proliferation and dacarbazine insensitivity
| ||
J Cell Biol The modified mitochondrial outer membrane carrier MTCH2 links mitochondrial fusion to lipogenesis. | ||
FASEB J MTCH2 promotes adipogenesis in intramuscular preadipocytes via an m6A-YTHDF1-dependent mechanism. | ||
RNA Biol Reprogramming of m6A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia. |