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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTA3 Recombinant monoclonal antibody (83042-3-RR)

    WB analysis using 83042-3-RR (same clone as 83042-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83042-3-RR (MTA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MTA3 Recombinant monoclonal antibody (83042-3-RR)

    WB analysis of MCF-7 using 83042-3-RR (same clone as 83042-3-PBS)

    WB result of MTA3 antibody (83042-3-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTA3 transfected MCF-7 cells. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP00017-2

    Sandwich ELISA sample test of MP00017-2

    Hela cell lysate was measured. The human MTA3 concentration of detected samples was determined to be 50.22 ng/mL (based on a 3.2 mg/mL extract load).

  • Sandwich ELISA standard curve of MP00017-2

    Sandwich ELISA standard curve of MP00017-2

    Sandwich ELISA standard curve of MP00017-2, Human MTA3 Recombinant Matched Antibody Pair - PBS only. 83042-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6400. 83042-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MTA3 Recombinant monoclonal antibody (83042-3-RR)

    IF Staining of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (83042-3-RR, Clone: 230350D12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using MTA3 Recombinant monoclonal antibody (83042-3-RR)

    FC experiment of U-251 using 83042-3-RR (same clone as 83042-3-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MTA3 Recombinant antibody (83042-3-RR, Clone:230350D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83042-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve of MP00017-2

    Cytometric bead array standard curve of MP00017-2, MTA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83042-3-PBS. Detection antibody: 83042-1-PBS. Standard: Ag6400. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83042-3-PBS

    Affinity and Kinetic Characterization of 83042-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83042-3-PBS against Human MTA3 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83042-3-RR

    Affinity and Kinetic Characterization of 83042-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83042-3-RR against Human MTA3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

MTA3 Recombinant monoclonal antibody, PBS Only (Capture)

MTA3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 83042-3-PBS
Clone No.230350D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

230350D12, Metastasis-associated protein MTA3, MTA 3, mta1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83042-3-PBS targets MTA3 as part of a matched antibody pair:

MP00017-2: 83042-3-PBS capture and 83042-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6400

Product name: Recombinant human MTA3 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-343 aa of BC053631

Sequence: MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISNTLIMLADKHAKEIEEESETTVEADLTDKQKHQLKHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPSLKTLLADKGEIRVGPRYQADIPEMLLEGESDEREQSKLEVKVWDPNSPLTDRQIDQFLVVARAVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLYRHSYDLSSAISVLVPLGGPVLCRDEMEEWSASEASLFEEALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYS

相同性解析による交差性が予測される生物種
Full Name metastasis associated 1 family, member 3
Calculated molecular weight 68 kDa
Observed molecular weight62 kDa, 68 kDa
GenBank accession numberBC053631
Gene Symbol MTA3
Gene ID (NCBI) 57504
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9BTC8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

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