Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, MCF-7 cells, Jurkat cells, A549 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HeLa cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87979-1-RR targets MTA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag30994 Product name: Recombinant human MTA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 513-678 aa of NM_004689 Sequence: TARLPEASQSPLVLKQAVRKPLEAVLRYLETHPRPPKPDPVKSVSSVLSSLTPAKVAPVINNGSPTILGKRSYEQHNGVDGNMKKRLLMPSRGLANHGQARHMGPSRNLLLNGKSYPTKVRLIRGGSLPPVKRRRMNWIDAPDDVFYMATEETRKIRKLLSSSETK 相同性解析による交差性が予測される生物種 |
| Full Name | metastasis associated 1 |
| Calculated molecular weight | 81 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | NM_004689 |
| Gene Symbol | MTA1 |
| Gene ID (NCBI) | 9112 |
| RRID | AB_3745844 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13330 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Metastasis-associated protein 1 (MTA1) is a member of the MTA family, which is involved in regulating DNA-based biological processes (PMID: 25332144). MTA1 is an integral part of the nucleosome remodeling histone deacetylase complex (NuRD complex) and regulates nucleosome remodeling and deacetylation by stabilizing the NuRD complex and related proteins (PMID: 12419236). MTA1 expression is associated with tumor malignancy in several cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTA1 antibody 87979-1-RR | Download protocol |
| WB protocol for MTA1 antibody 87979-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |