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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSX2 Monoclonal antibody (68550-1-Ig)

    WB analysis using 68550-1-Ig (same clone as 68550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68550-1-Ig (MSX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68550-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MSX2 Monoclonal antibody (68550-1-Ig)

    WB analysis using 68550-1-Ig (same clone as 68550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68550-1-Ig (MSX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MSX2 Monoclonal antibody (68550-1-Ig)

    IHC staining of human placenta using 68550-1-Ig (same clone as 68550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68550-1-Ig (MSX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68550-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MSX2 Monoclonal antibody (68550-1-Ig)

    IHC staining of human placenta using 68550-1-Ig (same clone as 68550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68550-1-Ig (MSX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68550-1-PBS in a different storage buffer formulation.

MSX2 Monoclonal antibody, PBS Only

MSX2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68550-1-PBS
Clone No.1B1G3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

1B1G3, Homeobox protein Hox-8, Homeobox protein MSX-2, HOX8

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68550-1-PBS targets MSX2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33220

Product name: Recombinant human MSX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-267 aa of BC015509

Sequence: MASPSKGNDLFSPDEEGPAVVAGPGPGPGGAEGAAEERRVKVSSLPFSVEALMSDKKPPKEASPLPAESASAGATLRPLLLSGHGAREAHSPGPLVKPFETASVKSENSEDGAAWMQEPGRYSPPPRHTSPTTCTLRKHKTNRKPRTPFTTSQLLALERKFRQKQYLSIAERAEFSSSLNLTETQVKIWFQNRRAKAKRLQEAELEKLKMAAKPMLPSSFSLPFPISSPLQAASIYGASYPFHRPVLPIPPVGLYATPVGYGMYHLS

相同性解析による交差性が予測される生物種
Full Name msh homeobox 2
Calculated molecular weight 29 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC015509
Gene Symbol MSX2
Gene ID (NCBI) 4488
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP35548
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MSX2 (Msh Homeobox 2) is a transcription factor belonging to the MSX homeobox gene family, which plays crucial roles in embryonic development, cell differentiation, and tissue homeostasis. The apparent molecular weight is larger than the calculated molecular weight of 29 kDa, which is likely due to posttranslational modification.

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