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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSTO1 Polyclonal antibody (28400-1-AP)

    WB analysis using 28400-1-AP (same clone as 28400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28400-1-AP (MSTO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MSTO1 Polyclonal antibody (28400-1-AP)

    WB analysis of HepG2 using 28400-1-AP (same clone as 28400-1-PBS)

    WB result of MSTO1 antibody (28400-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSTO1 transfected HepG2 cells. This data was developed using the same antibody clone with 28400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MSTO1 Polyclonal antibody (28400-1-AP)

    IHC staining of human ovary cancer using 28400-1-AP (same clone as 28400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28400-1-AP (MSTO1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MSTO1 Polyclonal antibody (28400-1-AP)

    IHC staining of human ovary cancer using 28400-1-AP (same clone as 28400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28400-1-AP (MSTO1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28400-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MSTO1 Polyclonal antibody, PBS Only

MSTO1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28400-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

SLTP005, MST, MMYAT, LST005

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28400-1-PBS targets MSTO1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29067

Product name: Recombinant human MSTO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 28-256 aa of BC002535

Sequence: QDAALGRATDSKEPPGELCPDVLYRTGRTLHGQETYTPRLILMDLKGSLSSLKEEGGLYRDKQLDAAIAWQGKLTTHKEELYPKNPYLQDFLSAEGVLSSDGVWRVKSIPNGKGSSPLPTATTPKPLIPTEASIRVWSDFLRVHLHPRSICMIQKYNHDGEAGRLEAFGQGESVLKEPKYQEELEDRLHFYVEECDYLQGFQILCDLHDGFSGVGAKAAELLQDEYSGR

相同性解析による交差性が予測される生物種
Full Name misato homolog 1 (Drosophila)
Observed molecular weight 57-62 kDa
GenBank accession numberBC002535
Gene Symbol MSTO1
Gene ID (NCBI) 55154
RRIDAB_2881133
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUK6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MSTO1, also named as LST005 and SLTP005, belongs to the misato family. Human MSTO1 is a ubiquitously expressed, evolutionary conserved protein with homologous regions to GTPases. MSTO1 is a cytoplasmic protein that modulates mitochondrial dynamics by promoting mitochondrial fusion. MSTO1 has 7 isoforms produced by alternative splicing. (PMID: 33612823)

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