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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSMB Polyclonal antibody (15888-1-AP)

    WB analysis using 15888-1-AP

    Human urine samples were subjected to SDS PAGE followed by western blot with 15888-1-AP (MSMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human lung using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human lung using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human prostate cancer using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human prostate cancer using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human prostate hyperplasia using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human prostate hyperplasia using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human kidney using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MSMB Polyclonal antibody (15888-1-AP)

    IHC staining of human kidney using 15888-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15888-1-AP (MSMB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of LNCaP cells using MSMB Polyclonal antibody (15888-1-AP)

    FC experiment of LNCaP using 15888-1-AP

    1x10^6 LNCaP cells were intracellularly stained with 0.25 ug MSMB Polyclonal antibody (15888-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

MSMB Polyclonal antibody

MSMB Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 15888-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Beta microseminoprotein, Beta-microseminoprotein, HPC13, Immunoglobulin-binding factor, PSP-94

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman urine sample
Positive IHC detected inhuman lung tissue, human prostate hyperplasia tissue, human kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15888-1-AP targets MSMB in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8674

Product name: Recombinant human MSMB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-114 aa of BC005257

Sequence: IPNEGVPGDSTRKCMDLKGNKHPINSEWQTDNCETCTCYETEISCCTLVSTPVGYDKDNCQRIFKKEDCKYIVVEKKDPKKTCSVSEWII

相同性解析による交差性が予測される生物種
Full Name microseminoprotein, beta-
Calculated molecular weight 114 aa, 13 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC005257
Gene Symbol MSMB
Gene ID (NCBI) 4477
RRIDAB_2878197
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP08118
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for MSMB antibody 15888-1-APDownload protocol
IHC protocol for MSMB antibody 15888-1-APDownload protocol
WB protocol for MSMB antibody 15888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Nutr Food Res

Prolonged Fructose Overconsumption Activates Original Biosynthetic and Metabolic Pathways of Endogenous Purine in Rats

Authors - Qiuxia Yu
View Article
humanWB,IHC

Front Oncol

WGCNA-ML-MR integration: uncovering immune-related genes in prostate cancer

Authors - Jing Lv
View Article
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