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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Polyclonal antibody (15600-1-AP)

    IHC staining of human ovary tumor using 15600-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Mesothelin Polyclonal antibody (15600-1-AP)

    IHC staining of human ovary tumor using 15600-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Mesothelin Polyclonal antibody (15600-1-AP)

    IHC staining of human pancreas cancer using 15600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Mesothelin Polyclonal antibody (15600-1-AP)

    IHC staining of human pancreas cancer using 15600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15600-1-AP (mesothelin antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Mesothelin Polyclonal antibody

Mesothelin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15600-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CAK1, CAK1 antigen, mesothelin, MPF, MPF Mesothelin, cleaved form, MSLN, SMR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman ovary tumor tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15600-1-AP targets Mesothelin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mesothelin fusion protein Ag7981 相同性解析による交差性が予測される生物種
Full Name mesothelin
Calculated molecular weight 69 kDa
GenBank accession numberBC003512
Gene Symbol MSLN
Gene ID (NCBI) 10232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13421
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

Protocols

Product Specific Protocols
IHC protocol for Mesothelin antibody 15600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols