Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, MOLT-4 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23601-1-AP targets MSL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18727 Product name: Recombinant human MSL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 228-577 aa of BC093790 Sequence: TEDLSDSLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETVSNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSEKVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSHSKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKACLDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAASTHDDKSLDEAIDMRFDC 相同性解析による交差性が予測される生物種 |
| Full Name | male-specific lethal 2 homolog (Drosophila) |
| Calculated molecular weight | 577 aa, 63 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC093790 |
| Gene Symbol | MSL2 |
| Gene ID (NCBI) | 55167 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9HCI7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
In mammals, MSL2 participates in histone H2B ubiquitination (H2Bub1) via the MSL complex, regulating transcription and chromatin remodeling. It is essential for embryonic development and has been implicated in cancer progression. MSL2's dysfunction is linked to developmental defects and oncogenesis, highlighting its critical role in gene regulation. (PMID: 30669413; PMID: 34899909)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MSL2 antibody 23601-1-AP | Download protocol |
| WB protocol for MSL2 antibody 23601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |