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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSK1 Polyclonal antibody (15977-1-AP)

    WB analysis using 15977-1-AP (same clone as 15977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15977-1-AP (MSK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MSK1 Polyclonal antibody (15977-1-AP)

    IHC staining of human lung cancer using 15977-1-AP (same clone as 15977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15977-1-AP (MSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MSK1 Polyclonal antibody (15977-1-AP)

    IHC staining of human lung cancer using 15977-1-AP (same clone as 15977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15977-1-AP (MSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15977-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MSK1 Polyclonal antibody (15977-1-AP)

    IF Staining of MCF-7 using 15977-1-AP (same clone as 15977-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MSK1 antibody (15977-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15977-1-PBS in a different storage buffer formulation.

MSK1 Polyclonal antibody, PBS Only

MSK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15977-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RPS6KA5, EC:2.7.11.1, MSPK1, Nuclear mitogen- and stress-activated protein kinase 1, Ribosomal protein S6 kinase alpha-5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15977-1-PBS targets MSK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8765

Product name: Recombinant human RPS6KA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-319 aa of BC017187

Sequence: MEEEGGSSGGAAGTSADGGDGGEQLLTVKHELRTANLTGHAEKVGIENFELLKVLGTGAYGKVFLVRKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDSNGHVVLTDFGLSKEFVADETERAYSFCGTIEYMAPDIVRGGDSGHDKAVDWWSLGVLMYELLTGASPFTVDGEKNSQAEISRRILKSEPPYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQ

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 5
Calculated molecular weight549aa,62 kDa; 802aa,90 kDa
Observed molecular weight 82-90 kDa
GenBank accession numberBC017187
Gene Symbol MSK1
Gene ID (NCBI) 9252
RRIDAB_2181799
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75582
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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