• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MSH3 Recombinant antibody (82845-3-RR)

    WB analysis using 82845-3-RR (same clone as 82845-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MSH3 Recombinant antibody (82845-3-RR)

    WB analysis of HEK-293 using 82845-3-RR (same clone as 82845-3-PBS)

    WB result of MSH3 antibody (82845-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH3 transfected HEK-293 cells. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MSH3 Recombinant antibody (82845-3-RR)

    IP experiment of HeLa using 82845-3-RR (same clone as 82845-3-PBS)

    IP result of anti-MSH3 (IP:82845-3-RR, 4ug; Detection:82845-3-RR 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MSH3 Recombinant antibody, PBS Only (82845-3-PBS)

    IF Staining of HeLa using 82845-3-PBS

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH3 antibody (82845-3-PBS, Clone: 1J23 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U2OS using 82845-3-RR (same clone as 82845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82845-3-RR

    Affinity and Kinetic Characterization of 82845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

MSH3 Recombinant monoclonal antibody, PBS Only

MSH3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82845-3-PBS
Clone No.1J23

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

1J23, Divergent upstream protein, DUC1, DUG, hMSH3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82845-3-PBS targets MSH3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18060

Product name: Recombinant human MSH3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC130434

Sequence: MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAATAPPAPPAPAFPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEFCCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSHENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKL

相同性解析による交差性が予測される生物種
Full Name mutS homolog 3 (E. coli)
Calculated molecular weight 1137 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC130434
Gene Symbol MSH3
Gene ID (NCBI) 4437
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20585
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

Protocols

Product Specific Protocols
IF protocol for MSH3 antibody 82845-3-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...