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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSH2 Recombinant antibody (82775-1-RR)

    WB analysis using 82775-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82775-1-RR (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MSH2 Recombinant antibody (82775-1-RR)

    WB analysis of HeLa using 82775-1-RR

    WB result of MSH2 antibody (82775-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human colon cancer using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human colon cancer using 82775-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82775-1-RR (MSH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human colon using 82775-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human colon using 82775-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human tonsillitis using 82775-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MSH2 Recombinant monoclonal antibody (82775-1-RR)

    IF Staining of HeLa using 82775-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody (82775-1-RR, Clone: 6O4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82775-1-RR

    Affinity and Kinetic Characterization of 82775-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82775-1-RR against Human MSH2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

MSH2 Recombinant monoclonal antibody

MSH2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82775-1-RR
Clone No.6O4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

6O4, MSH 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Caco-2 cells, A549 cells, HCT 116 cells
Positive IHC detected inhuman colon tissue, human colon cancer, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82775-1-RR targets MSH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7835

Product name: Recombinant human MSH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 585-934 aa of BC021566

Sequence: SSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAPVPYVRPAILEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLETASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFCMFATHFHELTALANQIPTVNNLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHVIECAKQKALELEEFQYIGESQGYDIMEPAAKKCYLEREQGEKIIQEFLSKVKQMPFTEMSEENITIKLKQLKAEVIAKNNSFVNEIISRIKVTT

相同性解析による交差性が予測される生物種
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight 100 kDa-105 kDa
GenBank accession numberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
RRIDAB_3086533
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP43246
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Protocols

Product Specific Protocols
WB protocol for MSH2 antibody 82775-1-RRDownload protocol
IHC protocol for MSH2 antibody 82775-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols