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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MSC Polyclonal antibody

MSC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25028-1-AP

Synonyms

ABF 1, ABF1, Activated B cell factor 1, bHLHa22, MSC, Musculin, MYOR

Validation Data Gallery

Applications:
  • WB analysis of mouse spleen using 25028-1-AP

    WB analysis of mouse spleen using 25028-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 25028-1-AP

    WB analysis of mouse thymus using 25028-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 25028-1-AP

    IHC staining of human lymphoma using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 25028-1-AP

    IHC staining of human tonsillitis using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 25028-1-AP

    IHC staining of human lymphoma using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 25028-1-AP

    IHC staining of human tonsillitis using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25028-1-AP is MSC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSC fusion protein Ag13489 相同性解析による交差性が予測される生物種
Full Name musculin (activated B-cell factor-1)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC006313
Gene symbol MSC
Gene ID (NCBI) 9242
RRIDAB_2879857
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSC, also named as Musculin, is a 206 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. MSC localizes in the nucleus and as a transcription repressor is capable of inhibiting the transactivation capability of TCF3/E47. MSC may play a role in regulating antigen-dependent B-cell differentiation.

Protocols

Product Specific Protocols
WB protocol for MSC antibody 25028-1-APDownload protocol
IHC protocol for MSC antibody 25028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols