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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using MSC Polyclonal antibody (25028-1-AP)

    WB analysis of mouse spleen using 25028-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using MSC Polyclonal antibody (25028-1-AP)

    WB analysis of mouse thymus using 25028-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MSC Polyclonal antibody (25028-1-AP)

    IHC staining of human lymphoma using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MSC Polyclonal antibody (25028-1-AP)

    IHC staining of human lymphoma using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MSC Polyclonal antibody

MSC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25028-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

bHLHa22, Activated B-cell factor 1, Activated B cell factor 1, ABF-1, ABF1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25028-1-AP targets MSC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13489

Product name: Recombinant human MSC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-206 aa of BC006313

Sequence: MSTGSVSDPEEMELRGLQREYPVPASKRPPLRGVERSYASPSDNSSAEEEDPDGEEERCALGTAGSAEGCKRKRPRVAGGGGAGGSAGGGGKKPLPAKGSAAECKQSQRNAANARERARMRVLSKAFSRLKTSLPWVPPDTKLSKLDTLRLASSYIAHLRQLLQEDRYENGYVHPVNLTWPFVVSGRPDSDTKEVSAANRLCGTTA

相同性解析による交差性が予測される生物種
Full Name musculin (activated B-cell factor-1)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC006313
Gene Symbol MSC
Gene ID (NCBI) 9242
RRIDAB_2879857
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60682
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSC, also named as Musculin, is a 206 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. MSC localizes in the nucleus and as a transcription repressor is capable of inhibiting the transactivation capability of TCF3/E47. MSC may play a role in regulating antigen-dependent B-cell differentiation.

Protocols

Product Specific Protocols
IHC protocol for MSC antibody 25028-1-APDownload protocol
WB protocol for MSC antibody 25028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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