Validation Data Gallery
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Tested Applications
| Positive WB detected in | human myotube protein, HeLa cells, mouse liver mitochondria tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
18441-1-AP targets MRPS30 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13271 Product name: Recombinant human MRPS30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 89-439 aa of BC007735 Sequence: MKYMVYPQTFALNADRWYQYFTKTVFLSGLPPPPAEPEPEPEPEPEPALDLAALRAVACDCLLQEHFYLRRRRRVHRYEESEVISLPFLDQLVSTLVGLLSPHNPALAAAALDYRCPVHFYWVRGEEIIPRGHRRGRIDDLRYQIDDKPNNQIRISKQLAEFVPLDYSVPIEIPTIKCKPDKLPLFKRQYENHIFVGSKTADPCCYGHTQFHLLPDKLRRERLLRQNCADQIEVVFRANAIASLFAWTGAQAMYQGFWSEADVTRPFVSQAVITDGKYFSFFCYQLNTLALTTQADQNNPRKNICWGTQSKPLYETIEDNDVKGFNDDVLLQIVHFLLNRPKEEKSQLLEN 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein S30 |
| Calculated molecular weight | 439 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC007735 |
| Gene Symbol | MRPS30 |
| Gene ID (NCBI) | 10884 |
| RRID | AB_2878544 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NP92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPS30 antibody 18441-1-AP | Download protocol |
| WB protocol for MRPS30 antibody 18441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation | ||
Nat Commun P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance |
