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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS18A Recombinant antibody (84201-1-RR)

    WB analysis using 84201-1-RR (same clone as 84201-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84201-1-RR (MRPS18A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84201-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MRPS18A Recombinant antibody (84201-1-RR)

    WB analysis of HepG2 using 84201-1-RR (same clone as 84201-1-PBS)

    WB result of MRPS18A antibody (84201-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS18A transfected HepG2 cells. This data was developed using the same antibody clone with 84201-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84201-1-RR

    Affinity and Kinetic Characterization of 84201-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84201-1-RR against Human MRPS18A were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

MRPS18A Recombinant monoclonal antibody, PBS Only

MRPS18A Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 84201-1-PBS
Clone No.240850C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial, 39S ribosomal protein S18-3, mitochondrial

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84201-1-PBS targets MRPS18A in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9011

Product name: Recombinant human MRPS18A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-196 aa of BC010364

Sequence: MAALKALVSGCGRLLRGLLAGPAATSWSRLPARGFREVVETQEGKTTIIEGRITATPKESPNPPNPSGQCPICRWNLKHKYNYDDVLLLSQFIRPHGGMLPRKITGLCQEEHRKIEECVKMAHRAGLLPNHRPRLPEGVVPKSKPQLNRYLTRWAPGSVKPIYKKGPRWNRVRMPVGSPLLRDNVCYSRTPWKLYH

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S18A
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight15 kDa
GenBank accession numberBC010364
Gene Symbol MRPS18A
Gene ID (NCBI) 55168
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9NVS2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MRPS18A (Mitochondrial Ribosomal Protein S18A) is a protein coding gene. It encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The increased expression of MRPS18A in cancer cells is explained by the increased energy metabolism of cancer cells and may pave the way for new diagnostic and therapeutic avenues to be explored (PMID: 28056857).