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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MRPL55 Polyclonal antibody (17679-1-AP)

    WB analysis of HEK-293 using 17679-1-AP (same clone as 17679-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17679-1-AP (MRPL55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17679-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MRPL55 Polyclonal antibody (17679-1-AP)

    IP experiment of HEK-293 using 17679-1-AP (same clone as 17679-1-PBS)

    IP result of anti-MRPL55 (IP:17679-1-AP, 4ug; Detection:17679-1-AP 1:600) with HEK-293 cells lysate 1680 ug. This data was developed using the same antibody clone with 17679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MRPL55 Polyclonal antibody (17679-1-AP)

    IHC staining of human colon cancer using 17679-1-AP (same clone as 17679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MRPL55 Polyclonal antibody (17679-1-AP)

    IHC staining of human colon cancer using 17679-1-AP (same clone as 17679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL55 Polyclonal antibody (17679-1-AP)

    IHC staining of human kidney using 17679-1-AP (same clone as 17679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL55 Polyclonal antibody (17679-1-AP)

    IHC staining of human kidney using 17679-1-AP (same clone as 17679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17679-1-PBS in a different storage buffer formulation.

MRPL55 Polyclonal antibody, PBS Only

MRPL55 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17679-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

MRP L55, Mitochondrial large ribosomal subunit protein bL31m, Large ribosomal subunit protein mL55, L55nt, L55mt

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17679-1-PBS targets MRPL55 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11716

Product name: Recombinant human MRPL55 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC052806

Sequence: MAAVGSLLGRLRQSTVKATGPALRRLHTSSWRADSSRASLTRVHRQAYARLYPVLLVKQDGSTIHIRYREPRRMLAMPIDLDTLSPEERRARLRKREAQLQSRKEYEQELSDDLHVERYRQFWTRTKK

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L55
Calculated molecular weight 128 aa, 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC052806
Gene Symbol MRPL55
Gene ID (NCBI) 128308
RRIDAB_2878427
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z7F7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MRPL55, also named as L55mt and MRP-L55, is a component of the mitochondrial ribosome large subunit (39S). It acts dynamically in the cell during development. The MW of MRPL55 is about 13 kDa.

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