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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    WB analysis of mouse liver using 17683-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17683-1-AP (MRPL54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human skin cancer using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human skin cancer using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human oesophagus using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human oesophagus using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL54 Polyclonal antibody (17683-1-AP)

    IF Staining of HeLa using 17683-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17683-1-AP (MRPL54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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MRPL54 Polyclonal antibody

MRPL54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17683-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MRP-L54, MRP L54, Large ribosomal subunit protein mL54, L54mt

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman skin cancer tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17683-1-AP targets MRPL54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL54 fusion protein Ag11782 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L54
Calculated molecular weight 138 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC065273
Gene Symbol MRPL54
Gene ID (NCBI) 116541
RRIDAB_2878429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL54 antibody 17683-1-APDownload protocol
IHC protocol for MRPL54 antibody 17683-1-APDownload protocol
IF protocol for MRPL54 antibody 17683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols