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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL52 Polyclonal antibody

MRPL52 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16800-1-AP

Synonyms

L52mt, MRP L52, MRPL52

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 16800-1-AP

    WB analysis of HeLa using 16800-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16800-1-AP (MRPL52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 16800-1-AP

    WB analysis of human heart using 16800-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16800-1-AP (MRPL52 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16800-1-AP

    IHC staining of human kidney using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 16800-1-AP

    IHC staining of human kidney using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 16800-1-AP

    IHC staining of human testis using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 16800-1-AP

    IHC staining of human testis using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human spleen using 16800-1-AP

    IHC staining of human spleen using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 16800-1-AP

    IHC staining of human spleen using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16800-1-AP

    IF Staining of HepG2 using 16800-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16800-1-AP targets MRPL52 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL52 fusion protein Ag10280 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L52
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC068070
Gene symbol MRPL52
Gene ID (NCBI) 122704
RRIDAB_2250853
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL52 antibody 16800-1-APDownload protocol
IHC protocol for MRPL52 antibody 16800-1-APDownload protocol
IF protocol for MRPL52 antibody 16800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Oral Pathol Med

LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.

Authors - Xiuhe Zou
View Article