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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using MRPL52 Polyclonal antibody (16800-1-AP)

    WB analysis of human heart using 16800-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16800-1-AP (MRPL52 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MRPL52 Polyclonal antibody (16800-1-AP)

    WB analysis of HeLa using 16800-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16800-1-AP (MRPL52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL52 Polyclonal antibody (16800-1-AP)

    IHC staining of human kidney using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL52 Polyclonal antibody (16800-1-AP)

    IHC staining of human kidney using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPL52 Polyclonal antibody (16800-1-AP)

    IHC staining of human testis using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPL52 Polyclonal antibody (16800-1-AP)

    IHC staining of human testis using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MRPL52 Polyclonal antibody (16800-1-AP)

    IHC staining of human spleen using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MRPL52 Polyclonal antibody (16800-1-AP)

    IHC staining of human spleen using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL52 Polyclonal antibody (16800-1-AP)

    IF Staining of HepG2 using 16800-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MRPL52 Polyclonal antibody

MRPL52 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 16800-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

L52mt, MRP L52, MRPL52

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16800-1-AP targets MRPL52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10280

Product name: Recombinant human MRPL52 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-123 aa of BC068070

Sequence: MAALGTVLFTGVRRLHCSAAAWAGGQWRLQQGLAANPSGYGPLTELPDCSYADGRPAPPMKGQLRRKAERETFARRVVLLSQEMDAGLQAWQLRQQKLQEEQRKQENALKPKGASLKSPLPSQ

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L52
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC068070
Gene Symbol MRPL52
Gene ID (NCBI) 122704
RRIDAB_2250853
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TS9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for MRPL52 antibody 16800-1-APDownload protocol
IHC protocol for MRPL52 antibody 16800-1-APDownload protocol
WB protocol for MRPL52 antibody 16800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Oral Pathol Med

LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.

Authors - Xiuhe Zou
View Article
humanWB

Nat Struct Mol Biol

A roadmap for ribosome assembly in human mitochondria

Authors - Elena Lavdovskaia
View Article
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