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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    WB analysis using 83565-1-RR (same clone as 83565-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83565-1-RR (MRPL51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    WB analysis of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)

    WB result of MRPL51 antibody (83565-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL51 transfected HEK-293 cells. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IHC staining of human ovarian cancer using 83565-1-RR (same clone as 83565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IF Staining of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MRPL51 antibody (83565-1-RR, Clone: 240381C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83565-1-RR

    Affinity and Kinetic Characterization of 83565-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83565-1-RR against Human MRPL51 were performed. The affinity constant is 0.57 nM.

  • Featured Product
  • KD/KO Validated

MRPL51 Recombinant monoclonal antibody, PBS Only

MRPL51 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83565-1-PBS
Clone No.240381C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

240381C3, bMRP 64, bMRP64, bMRP-64, CDA09

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83565-1-PBS targets MRPL51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7193

Product name: Recombinant human MRPL51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC000191

Sequence: MAGNLLSGAGRRLWDWVPLACRSFSLGVPRLIGIRLTLPPPKVVDRWNEKRAMFGVYDNIGILGNFEKHPKELIRGPIWLRGWKGNELQRCIRKRKMVGSRMFADDLHNLNKRIRYLYKHFNRHGKFR

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L51
Calculated molecular weight 15 kDa
Observed molecular weight12 kDa
GenBank accession numberBC000191
Gene Symbol MRPL51
Gene ID (NCBI) 51258
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ4U2R6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial ribosome protein L51 (MRPL51) is a 39S subunit protein of the mitochondrial ribosome. MRPL51 functions in the translation of genetic messages (PMID: 21730110). MRPL51 expression was positively correlated with cell cycle, DNA damage, DNA repair, epithelial-mesenchymal transition, invasion, and proliferation of LUAD cells at the single cell level (PMID: 37323822). The calculated molecular weight of MRPL51 is 15 kDa.

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