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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    WB analysis using 83565-1-RR (same clone as 83565-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83565-1-RR (MRPL51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    WB analysis of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)

    WB result of MRPL51 antibody (83565-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL51 transfected HEK-293 cells. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IHC staining of human ovarian cancer using 83565-1-RR (same clone as 83565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MRPL51 Recombinant monoclonal antibody (83565-1-RR)

    IF Staining of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MRPL51 antibody (83565-1-RR, Clone: 240381C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83565-1-RR

    Affinity and Kinetic Characterization of 83565-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83565-1-RR against Human MRPL51 were performed. The affinity constant is 0.57 nM.

  • Featured Product
  • KD/KO Validated

MRPL51 Recombinant monoclonal antibody, PBS Only

MRPL51 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83565-1-PBS
Clone No.240381C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

240381C3, bMRP 64, bMRP64, bMRP-64, CDA09

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83565-1-PBS targets MRPL51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7193

Product name: Recombinant human MRPL51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC000191

Sequence: MAGNLLSGAGRRLWDWVPLACRSFSLGVPRLIGIRLTLPPPKVVDRWNEKRAMFGVYDNIGILGNFEKHPKELIRGPIWLRGWKGNELQRCIRKRKMVGSRMFADDLHNLNKRIRYLYKHFNRHGKFR

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L51
Calculated molecular weight 15 kDa
Observed molecular weight12 kDa
GenBank accession numberBC000191
Gene Symbol MRPL51
Gene ID (NCBI) 51258
RRIDAB_3671180
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ4U2R6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mitochondrial ribosome protein L51 (MRPL51) is a 39S subunit protein of the mitochondrial ribosome. MRPL51 functions in the translation of genetic messages (PMID: 21730110). MRPL51 expression was positively correlated with cell cycle, DNA damage, DNA repair, epithelial-mesenchymal transition, invasion, and proliferation of LUAD cells at the single cell level (PMID: 37323822). The calculated molecular weight of MRPL51 is 15 kDa.

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