• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis using 66531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis of HeLa using 66531-1-Ig

    WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells.

  • Western Blot (WB) analysis of RAW 264.7 cells using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis of RAW 264.7 using 66531-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis of HSC-T6 using 66531-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL45 Monoclonal antibody (66531-1-Ig)

    IHC staining of human breast cancer using 66531-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL45 Monoclonal antibody (66531-1-Ig)

    IHC staining of human breast cancer using 66531-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

MRPL45 Monoclonal antibody

MRPL45 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66531-1-Ig
Clone No.1B4B4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

L45mt, MRPL45, MRP-L45

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, RAW 264.7 cells, HEK-293 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66531-1-Ig targets MRPL45 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8207

Product name: Recombinant human MRPL45 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 86-329 aa of BC006235

Sequence: AGLVIPPEKSDRSIHLACTAGIFDAYVPPEGDARISSLSKEGLIERTERMKKTMASQVSIRRIKDYDANFKIKDFPEKAKDIFIEAHLCLNNSDHDRLHTLVTEHCFPDMTWDIKYKTVRWSFVESLEPSHVVQVRCSSMMNQGNVYGQITVRMHTRQTLAIYDRFGRLMYGQEDVPKDVLEYVVFEKQLTNPYGSWRMHTKIVPPWAPPKQPILKTVMIPGPQLKPEEEYEEAQGEAQKPQLA

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L45
Calculated molecular weight 35 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC006235
Gene Symbol MRPL45
Gene ID (NCBI) 84311
RRIDAB_2881894
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9BRJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.

Protocols

Product Specific Protocols
WB protocol for MRPL45 antibody 66531-1-IgDownload protocol
IHC protocol for MRPL45 antibody 66531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols