Validation Data Gallery
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Tested Applications
| Positive WB detected in | HL-60 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:40000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
27484-1-AP targets MRPL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26568 Product name: Recombinant human MRPL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-154 aa of BC000756 Sequence: SSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHG 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L4 |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC000756 |
| Gene Symbol | MRPL4 |
| Gene ID (NCBI) | 51073 |
| RRID | AB_2810294 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYD3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPL4 antibody 27484-1-AP | Download protocol |
| IHC protocol for MRPL4 antibody 27484-1-AP | Download protocol |
| WB protocol for MRPL4 antibody 27484-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Metab Tetracyclines promote survival and fitness in mitochondrial disease models.
| ||
bioRxiv Tetracycline-dependent inhibition of mitoribosome protein elongation in mitochondrial disease mutant cells suppresses IRE1α to promote cell survival | ||
EMBO Rep Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival | ||
Sci Rep Construction of molecular subtype and prognostic model for gastric cancer based on nucleus-encoded mitochondrial genes | ||