Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse liver using 15190-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15190-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 15190-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 15190-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15190-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15190-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 15190-1-AP (MRPL37 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL37 Polyclonal antibody

MRPL37 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15190-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

L2mt, L37mt, MRP L2, MRP L37, MRPL2, MRPL37, RPML2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, human heart tissue, mouse ovary tissue, mouse uterus tissue
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below
IHC	See 1 publications below

Product Information

15190-1-AP targets MRPL37 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7276 Product name: Recombinant human MRPL37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-423 aa of BC000041 Sequence: KQALWLTKTKLIEGLPEKVLSLVDDPRNHIENQDECVLNVISHARLWQTTEEIPKRETYCPVIVDNLIQLCKSQILKHPSLARRICVQNSTFSATWNRESLLLQVRGSGGARLSTKDPLPTIASREEIEATKNHVLETFYPISPIIDLHECNIYDVKNDTGFQEGYPYPYPHTLYLLDKANLRPHRLQPDQLRAKMILFAFGSALAQARLLYGNDAKVLEQPVVVQSVGTDGRVFHFLVFQLNTTDLDCNEGVKNLAWVDSDQLLYQHFWCLPVIKKRVVVEPVGPVGFKPETFRKFLALYLHGAA 相同性解析による交差性が予測される生物種
Full Name	mitochondrial ribosomal protein L37
Calculated molecular weight	48 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC000041
Gene Symbol	MRPL37
Gene ID (NCBI)	51253
RRID	AB_2146040
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZE1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL37 antibody 15190-1-AP	Download protocol
IHC protocol for MRPL37 antibody 15190-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Sci Adv Adult-onset obesity is triggered by impaired mitochondrial gene expression. Authors - Kara L Perks View Article
mouse	WB	Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. Authors - Danielle L Rudler View Article
mouse	WB	EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. Authors - Nicola Ferreira View Article
mouse	WB	Aging Cell Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan. Authors - Tara R Richman View Article
human	WB	Am J Hum Genet Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome. Authors - Nicole J Lake View Article
mouse	WB	EMBO Rep Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z. Authors - Stefan J Siira View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 15190-1-AP

WB analysis of mouse liver using 15190-1-AP

WB analysis of mouse ovary using 15190-1-AP

WB analysis of mouse uterus using 15190-1-AP

WB analysis of human heart using 15190-1-AP

WB analysis of human heart using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

MRPL37 Polyclonal antibody

MRPL37 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.

Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.

Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.