Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse liver using 15190-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15190-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15190-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15190-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 15190-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse uterus using 15190-1-AP
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 15190-1-AP (MRPL37 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 15190-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL37 Polyclonal antibody

MRPL37 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15190-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HSPC235, L2mt, L37mt, Large ribosomal subunit protein mL37, MRP L2

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, human heart tissue, mouse ovary tissue, mouse uterus tissue
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 1 publications below

Product Information

15190-1-AP targets MRPL37 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7276 Product name: Recombinant human MRPL37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-423 aa of BC000041 Sequence: KQALWLTKTKLIEGLPEKVLSLVDDPRNHIENQDECVLNVISHARLWQTTEEIPKRETYCPVIVDNLIQLCKSQILKHPSLARRICVQNSTFSATWNRESLLLQVRGSGGARLSTKDPLPTIASREEIEATKNHVLETFYPISPIIDLHECNIYDVKNDTGFQEGYPYPYPHTLYLLDKANLRPHRLQPDQLRAKMILFAFGSALAQARLLYGNDAKVLEQPVVVQSVGTDGRVFHFLVFQLNTTDLDCNEGVKNLAWVDSDQLLYQHFWCLPVIKKRVVVEPVGPVGFKPETFRKFLALYLHGAA 相同性解析による交差性が予測される生物種
Full Name	mitochondrial ribosomal protein L37
Calculated molecular weight	48 kDa
Observed molecular weight	48 kDa
GenBank accession number	BC000041
Gene Symbol	MRPL37
Gene ID (NCBI)	51253
RRID	AB_2146040
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZE1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for MRPL37 antibody 15190-1-AP	Download protocol
WB protocol for MRPL37 antibody 15190-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes Authors - Wenyuan Wu View Article
human	WB	Nat Commun P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance Authors - Xiangli Jiang View Article
human	WB	Mol Cell Microprotein SMIM26 drives oxidative metabolism via serine-responsive mitochondrial translation Authors - Jiemin Nah View Article
mouse	WB	Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. Authors - Danielle L Rudler View Article
mouse	WB	Sci Adv Adult-onset obesity is triggered by impaired mitochondrial gene expression. Authors - Kara L Perks View Article
mouse	WB	EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. Authors - Nicola Ferreira View Article

Validation Data Gallery

WB analysis of mouse liver using 15190-1-AP

WB analysis of mouse liver using 15190-1-AP

WB analysis of human heart using 15190-1-AP

WB analysis of human heart using 15190-1-AP

WB analysis of mouse ovary using 15190-1-AP

WB analysis of mouse uterus using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

IHC staining of human lymphoma using 15190-1-AP

MRPL37 Polyclonal antibody

MRPL37 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes

P53-dependent hypusination of eIF5A affects mitochondrial translation and senescence immune surveillance

Microprotein SMIM26 drives oxidative metabolism via serine-responsive mitochondrial translation

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

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