Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells, HeLa cells, HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85327-1-RR targets MRPL3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | MRPL3 fusion protein Ag9970 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L3 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 35-39 kDa |
| GenBank accession number | BC003375 |
| Gene Symbol | MRPL3 |
| Gene ID (NCBI) | 11222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09001 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRPL3, also named as 39S ribosomal protein L3, mitochondrial or RPML3, is a 348 amino acid protein, which belongs to the ribosomal protein L3P family. MRPL3 localizes in the Mitochondrion and has an association with combined oxidative phosphorylation deficiency 9. MRPL3 plays role in the mitochondrial proteins biosynthesis and ribosomal structure and biogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL3 antibody 85327-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |