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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL28 Monoclonal antibody (68467-1-Ig)

    WB analysis using 68467-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68467-1-Ig (MRPL28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL28 Monoclonal antibody (68467-1-Ig)

    IF Staining of HepG2 using 68467-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL28 antibody (68467-1-Ig, Clone: 2E6B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

MRPL28 Monoclonal antibody

MRPL28 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68467-1-Ig
Clone No.2E6B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

L28mt, Large ribosomal subunit protein bL28m, MAAT1, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, 4T1 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68467-1-Ig targets MRPL28 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33198

Product name: Recombinant human MRPL28 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-256 aa of BC000990

Sequence: MPLHKYPVWLWKRLQLREGICSRLPGYYLRSLEEERTPTPVHYRPHGAKFKINPKNGQRERVEDVPIPIYFPPESQRGLWGGEGWILGQIYANNDKLSKRLKKVWKPQLFEREFYSEILDKKFTVTVTMRTLDLIDEAYGLDFYILKTPKEDLCSKFGMDLKRGMLLRLARQDPQLHPEDPERRAAIYDKYKEFAIPEEEAEWVGLTLEEAIEKQRLLEEKDPVPLFKIYVAELIQQLQQQALSEPAVVQKRASGQ

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L28
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000990
Gene Symbol MRPL28
Gene ID (NCBI) 10573
RRIDAB_3085178
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for MRPL28 antibody 68467-1-IgDownload protocol
WB protocol for MRPL28 antibody 68467-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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