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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using MRPL27 Polyclonal antibody (14765-1-AP)

    WB analysis of BxPC-3 using 14765-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14765-1-AP (MRPL27 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MRPL27 Polyclonal antibody (14765-1-AP)

    IF Staining of A431 using 14765-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MRPL27 antibody (14765-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MRPL27 Polyclonal antibody

MRPL27 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 14765-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MRP-L27, MRPL 27, MRP L27, L27mt, HSPC250

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14765-1-AP targets MRPL27 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL27 fusion protein Ag6527 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L27
Calculated molecular weight 16 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001066
Gene Symbol MRPL27
Gene ID (NCBI) 51264
RRIDAB_2145890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL27 antibody 14765-1-APDownload protocol
IF protocol for MRPL27 antibody 14765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article