Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
16224-1-AP targets MRPL24 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9277 Product name: Recombinant human MRPL24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC016700 Sequence: MRLSALLALASKVTLPPHYRYGMSPPGSVADKRKNPPWIRRRPVVVEPISDEDWYLFCGDTVEILEGKDAGKQGKVVQVIRQRNWVVVGGLNTHYRYIGKTMDYRGTMIPSEAPLLHRQVKLVDPMDRKPTEIEWRFTEAGERVRVSTRSGRIIPKPEFPRADGIVPETWIDGPKDTSVEDALERTYVPCLKTLQEEVMEAMGIKETRKYKKVYWY 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L24 |
| Calculated molecular weight | 216 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC016700 |
| Gene Symbol | MRPL24 |
| Gene ID (NCBI) | 79590 |
| RRID | AB_2297885 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96A35 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MRPL24 antibody 16224-1-AP | Download protocol |
| WB protocol for MRPL24 antibody 16224-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. | ||
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation | ||
J Biol Chem The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules. | ||
Proteomes Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. | ||
3 Biotech MRPL24 drives breast cancer metastasis and stemness by targeting c-MYC, BRD4, and STAT3 | ||
