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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL12 Recombinant antibody (85070-5-RR)

    WB analysis using 85070-5-RR (same clone as 85070-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85070-5-RR (MRPL12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL12 Recombinant antibody (85070-5-RR)

    IF Staining of HepG2 using 85070-5-RR (same clone as 85070-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (85070-5-RR, Clone: 242622D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85070-5-RR

    Affinity and Kinetic Characterization of 85070-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85070-5-RR against Human MRPL12 were performed. The affinity constant is below 1 pM.

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MRPL12 Recombinant antibody, PBS Only

MRPL12 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85070-5-PBS
Clone No.242622D12

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242622D12, 5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85070-5-PBS targets MRPL12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MRPL12 fusion protein Ag6504 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L12
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC002344
Gene Symbol MRPL12
Gene ID (NCBI) 6182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52815
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression.