Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
85070-5-PBS targets MRPL12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6504 Product name: Recombinant human MRPL12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC002344 Sequence: MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L12 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC002344 |
| Gene Symbol | MRPL12 |
| Gene ID (NCBI) | 6182 |
| RRID | AB_3743988 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P52815 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression.