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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL11 Recombinant monoclonal antibody (85109-4-RR)

    IF Staining of HepG2 using 85109-4-RR (same clone as 85109-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85109-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL11 Recombinant monoclonal antibody (85109-4-RR)

    IF Staining of HeLa using 85109-4-RR (same clone as 85109-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85109-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using MRPL11 Recombinant monoclonal antibody (85109-4-RR)

    FC experiment of HepG2 using 85109-4-RR (same clone as 85109-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MRPL11 Recombinant antibody (85109-4-RR, Clone:242768G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85109-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85109-4-RR

    Affinity and Kinetic Characterization of 85109-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85109-4-RR against Human MRPL11 were performed. The affinity constant is 14.5 nM.

MRPL11 Recombinant monoclonal antibody, PBS Only

MRPL11 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85109-4-PBS
Clone No.242768G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242768G1, CGI 113, CGI-113, L11mt, Large ribosomal subunit protein uL11m

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85109-4-PBS targets MRPL11 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7899

Product name: Recombinant human MRPL11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-192 aa of BC005002

Sequence: MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKEADLAAQEEAAKK

相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L11
Calculated molecular weight 21 kDa
GenBank accession numberBC005002
Gene Symbol MRPL11
Gene ID (NCBI) 65003
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3B7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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