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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL11 Recombinant antibody (85109-4-RR)

    IF Staining of HepG2 using 85109-4-RR (same clone as 85109-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85109-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using MRPL11 Recombinant antibody (85109-4-RR)

    FC experiment of HepG2 using 85109-4-RR (same clone as 85109-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MRPL11 Recombinant antibody (85109-4-RR, Clone:242768G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85109-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85109-4-RR

    Affinity and Kinetic Characterization of 85109-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85109-4-RR against Human MRPL11 were performed. The affinity constant is 14.5 nM.

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MRPL11 Recombinant antibody, PBS Only

MRPL11 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85109-4-PBS
Clone No.242768G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MRP L11, Large ribosomal subunit protein uL11m, L11mt, CGI-113, CGI 113

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85109-4-PBS targets MRPL11 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MRPL11 fusion protein Ag7899 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L11
Calculated molecular weight 21 kDa
GenBank accession numberBC005002
Gene Symbol MRPL11
Gene ID (NCBI) 65003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3B7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.