Validation Data Gallery
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Tested Applications
| Positive WB detected in | BxPC-3 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 21 publications below |
| IF | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
15543-1-AP targets MRPL11 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7899 Product name: Recombinant human MRPL11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC005002 Sequence: MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKEADLAAQEEAAKK 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L11 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC005002 |
| Gene Symbol | MRPL11 |
| Gene ID (NCBI) | 65003 |
| RRID | AB_2297856 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3B7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRPL11 is one of the protein components of mitochondrial ribosomes encoded by the nuclear genome, which is essential for oxidative phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| WB protocol for MRPL11 antibody 15543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes | ||
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
J Exp Clin Cancer Res UBASH3B-mediated MRPL12 Y60 dephosphorylation inhibits LUAD development by driving mitochondrial metabolism reprogramming | ||