Validation Data Gallery
Tested Applications
| Positive WB detected in | 37°C incubated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27167-1-AP targets MRP4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25937 Product name: Recombinant human ABCC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 567-709 aa of BC041560 Sequence: AEVSRHLFELCICQILHEKITILVTHQLQYLKAASQILILKDGKMVQKGTYTEFLKSGIDFGSLLKKDNEESEQPPVPGTPTLRNRTFSESSVWSQQSSRPSLKDGALESQDTENVPVTLSEENRSEGKVGFQAYKNYFRAGA 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-binding cassette, sub-family C (CFTR/MRP), member 4 |
| Calculated molecular weight | 859 aa, 97 kDa |
| Observed molecular weight | 150-180 kDa |
| GenBank accession number | BC041560 |
| Gene Symbol | MRP4 |
| Gene ID (NCBI) | 10257 |
| RRID | AB_3085931 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15439 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRP4 (also known as ABCC4), belonging to ATP-binding cassette (ABC) transporter family, is a multi-transmembrane protein that is able to transport a range of organic anionic compounds (both endogenous and xenobiotic) out of the cell. It can exclude various drugs thus is considered as potential target to prevent drug-resistance.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRP4 antibody 27167-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
