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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HT-1080 using 19916-1-AP (same clone as 19916-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HL-60 using 19916-1-AP (same clone as 19916-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19916-1-PBS targets MRI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRI1 fusion protein Ag13809 相同性解析による交差性が予測される生物種
Full Name methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
Calculated molecular weight 369 aa, 39 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC001703
Gene Symbol MRI1
Gene ID (NCBI) 84245
RRIDAB_10638924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV20
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MRI1 is also named as MRDI and belongs to the eIF-2B alpha/beta/delta subunits family. MRI1 promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation (PMID:19620624). MRDI acts as a methylthioribose-1-phosphate (MTR-1-P) isomerase, catalyzing a critical step in methionine salvage. Second, MRDI promotes and is necessary for melanoma cell invasion, independent of its catalytic activity (PMID:23859498).