Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14207-1-AP targets MREG in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5416 Product name: Recombinant human MREG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-214 aa of BC032747 Sequence: MGLRDWLRTVCCCCGCECLEERALPEKEPLVSDNNPYSSFGATLVRDDEKNLWSMPHDVSHTEADDDRTLYNLIVIRNQQAKDSEEWQKLNYDIHTLRQVRREVRNRWKCILEDLGFQKEADSLLSVTKLSTISDSKNTRKAREMLLKLAEETNIFPTSWELSERYLFVVDRLIALDAAEEFFKLARRTYPKKPGVPCLADGQKELHYLPFPSP 相同性解析による交差性が予測される生物種 |
| Full Name | melanoregulin |
| Calculated molecular weight | 25kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC032747 |
| Gene Symbol | MREG |
| Gene ID (NCBI) | 55686 |
| RRID | AB_3085432 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N565 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MREG, also known as DSU, belongs to the melanoregulin family. MREG is a small, highly charged protein thought to play a role in organelle biogenesis due to its effect on pigmentation in dilute, ashen, and leaden mutant mice (PMID: 19240024). Additionally, MREG probably functions as a cargo-recognition protein that couples cytoplasmic vesicles to the transport machinery. MREG has 2 isoforms with the molecular weights of 25 and 26 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MREG antibody 14207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |