Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, mouse brain tissue, rat liver tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25513-1-AP targets MPZL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21905 Product name: Recombinant human MPZL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 32-235 aa of BC113586 Sequence: LEIRADAHVRGYVGEKIKLKCTFKSTSDVTDKLTIDWTYRPPSSSHTVSIFHYQSFQYPTTAGTFRDRISWVGNVYKGDASISISNPTIKDNGTFSCAVKNPPDVHHNIPMTELTVTERGFGTMLSSVALLSILVFVPSAVVVALLLVRMGRKAAGLKKRSRSGYKKSSIEVSDDTDQEEEEACMARLCVRCAECLDSDYEETY 相同性解析による交差性が予測される生物種 |
| Full Name | myelin protein zero-like 3 |
| Calculated molecular weight | 235 aa, 26 kDa |
| Observed molecular weight | 70 kDa, 28 kDa |
| GenBank accession number | BC113586 |
| Gene Symbol | MPZL3 |
| Gene ID (NCBI) | 196264 |
| RRID | AB_2880111 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UWV2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MPZL3 (Myelin protein zero-like protein 3) is a member of the myelin P0 protein family. The human MPZL3 gene is located at chromosome 11q23.3, and encodes a 235-amino acid polypeptide with an Immunoglobulin V-type (IgV) domain. MPZL3 protein is a membrane adhesion protein. It may be involved in immune function (PMID: 19054061). This polyclonal antibody raised against 32-235aa of human MPZL3 reveals bands of 28 kDa and 70 kDa, which could be endogenous MPZL3 of unmodified form and post-translational modified form, respectively (PMID: 17273165).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPZL3 antibody 25513-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
