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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using MPRIP Polyclonal antibody (20040-1-AP)

    WB analysis of SH-SY5Y using 20040-1-AP (same clone as 20040-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using MPRIP Polyclonal antibody (20040-1-AP)

    WB analysis of SKOV-3 using 20040-1-AP (same clone as 20040-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A2780 cells using MPRIP Polyclonal antibody (20040-1-AP)

    WB analysis of A2780 using 20040-1-AP (same clone as 20040-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SKOV-3 cells using MPRIP Polyclonal antibody (20040-1-AP)

    IP experiment of SKOV-3 using 20040-1-AP (same clone as 20040-1-PBS)

    IP result of anti-MPRIP (IP:20040-1-AP, 4ug; Detection:20040-1-AP 1:500) with SKOV-3 cells lysate 2000ug. This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using MPRIP Polyclonal antibody (20040-1-AP)

    IHC staining of human ovary using 20040-1-AP (same clone as 20040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using MPRIP Polyclonal antibody (20040-1-AP)

    IHC staining of human ovary using 20040-1-AP (same clone as 20040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using MPRIP Polyclonal antibody (20040-1-AP)

    IHC staining of mouse ovary using 20040-1-AP (same clone as 20040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20040-1-AP (MPRIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MPRIP Polyclonal antibody (20040-1-AP)

    IF Staining of mouse brain using 20040-1-AP (same clone as 20040-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed frozen OCT-embedded mouse brain tissue using 20040-1-AP (MPRIP antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MPRIP Polyclonal antibody (20040-1-AP)

    IF Staining of HeLa using 20040-1-AP (same clone as 20040-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MPRIP antibody (20040-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20040-1-PBS in a different storage buffer formulation.

MPRIP Polyclonal antibody, PBS Only

MPRIP Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, Indirect ELISA
Cat No. 20040-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, IP, Indirect ELISA

Synonyms

M RIP, MRIP, M-RIP, Myosin phosphatase Rho-interacting protein, p116Rip

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20040-1-PBS targets MPRIP in WB, IHC, IF/ICC, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name myosin phosphatase Rho interacting protein
Calculated molecular weight 117 kDa
Observed molecular weight 116-140 kDa
GenBank accession numberNM_015134
Gene Symbol MPRIP
Gene ID (NCBI) 23164
RRIDAB_10694433
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6WCQ1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.

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