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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPP5 Polyclonal antibody (17710-1-AP)

    WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MPP5 Polyclonal antibody (17710-1-AP)

    WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MPP5 Polyclonal antibody (17710-1-AP)

    WB analysis of human brain using 17710-1-AP (same clone as 17710-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MPP5 Polyclonal antibody (17710-1-AP)

    IP experiment of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    IP result of anti-MPP5 (IP:17710-1-AP, 3ug; Detection:17710-1-AP 1:1000) with mouse brain tissue lysate 8000ug. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPP5 Polyclonal antibody (17710-1-AP)

    IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPP5 Polyclonal antibody (17710-1-AP)

    IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MPP5 Polyclonal antibody (17710-1-AP)

    IF Staining of HEK-293 using 17710-1-AP (same clone as 17710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MPP5 antibody (17710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MPP5 Polyclonal antibody, PBS Only

MPP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17710-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17710-1-PBS targets MPP5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12010

Product name: Recombinant human PALS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-341 aa of BC095485

Sequence: MTTSHMNGHVTEESDSEVKNVDLASPEEHQKHREMAVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPMFDTEEGIVLESPHYAVKILEIEDLFSSLKHIQHTLVDSQSQEDISLLLQLVQNKDFQNAFKIHNAITVHMNKASPPFPLISNAQDLAQEVQTVLKPVHHKEGQELTALLNTPHIQALLLAHDKVAEQEMQLEPITDERVYESIGQYGGETVKIVRIEKARDIPLGATVRNEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPP

相同性解析による交差性が予測される生物種
Full Name membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
Calculated molecular weight 675 aa, 77 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC095485
Gene Symbol MPP5
Gene ID (NCBI) 64398
RRIDAB_2282012
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3R9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

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