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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using MPP2 Polyclonal antibody (13589-1-AP)

    WB analysis of mouse eye using 13589-1-AP (same clone as 13589-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13589-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MPP2 Polyclonal antibody (13589-1-AP)

    WB analysis using 13589-1-AP (same clone as 13589-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13589-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse eye tissue using MPP2 Polyclonal antibody (13589-1-AP)

    IP experiment of mouse eye using 13589-1-AP (same clone as 13589-1-PBS)

    IP result of anti-MPP2 (IP:13589-1-AP, 4ug; Detection:13589-1-AP 1:2000) with mouse eye tissue lysate 2260 ug. This data was developed using the same antibody clone with 13589-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPP2 Polyclonal antibody (13589-1-AP)

    IHC staining of mouse brain using 13589-1-AP (same clone as 13589-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13589-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPP2 Polyclonal antibody (13589-1-AP)

    IHC staining of mouse brain using 13589-1-AP (same clone as 13589-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13589-1-PBS in a different storage buffer formulation.

MPP2 Polyclonal antibody, PBS Only

MPP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13589-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13589-1-PBS targets MPP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4223

Product name: Recombinant human MPP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-552 aa of BC030287

Sequence: LLRNASGSVILKILPSYQEPHLPRQVFVKCHFDYDPARDSLIPCKEAGLRFNAGDLLQIVNQDDANWWQACHVEGGSAGLIPSQLLEEKRKAFVKRDLELTPNSGTLCGSLSGKKKKRMMYLTTKNAEFDRHELLIYEEVARMPPFRRKTLVLIGAQGVGRRSLKNKLIMWDPDRYGTTVPYTSRRPKDSEREGQGYSFVSRGEMEADVRAGRYLEHGEYEGNLYGTRIDSIRGVVAAGKVCVLDVNPQAVKVLRTAEFVPYVVFIEAPDFETLRAMNRAALESGISTKQLTEADLRRTVEESSRIQRGYGHYFDLCLVNSNLERTFRELQTAMEKLRTEPQWVPVSWVY

相同性解析による交差性が予測される生物種
Full Name membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Calculated molecular weight 576 aa, 65 kDa
Observed molecular weight 65 kDa, 60 kDa
GenBank accession numberBC030287
Gene Symbol MPP2
Gene ID (NCBI) 4355
RRIDAB_2935434
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14168
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Membrane protein palmitoylated 2 (MPP2) is a member of the membrane-associated guanylate kinase (MAGUK) family. MPPs can form diverse protein complexes at the cell membranes and play an important role in cell structure, polarity and cell adhesion. MPP2 is a novel postsynaptic scaffold protein that binds to the synaptic cell adhesion molecule SynCAM1. MPP2 also is a component of AMPA receptor-associated protein complexes.

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