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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using MPO Polyclonal antibody (22225-1-AP)

    WB analysis of HL-60 using 22225-1-AP (same clone as 22225-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22225-1-AP (MPO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using MPO Polyclonal antibody (22225-1-AP)

    WB analysis of U-937 using 22225-1-AP (same clone as 22225-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 22225-1-AP (MPO antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using MPO Polyclonal antibody (22225-1-AP)

    IP experiment of HL-60 using 22225-1-AP (same clone as 22225-1-PBS)

    IP result of anti-MPO (IP:22225-1-AP, 4ug; Detection:22225-1-AP 1:4000) with HL-60 cells lysate 1720 ug. This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MPO Polyclonal antibody (22225-1-AP)

    IHC staining of human colon cancer using 22225-1-AP (same clone as 22225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MPO Polyclonal antibody (22225-1-AP)

    IHC staining of human colon cancer using 22225-1-AP (same clone as 22225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using MPO Polyclonal antibody (22225-1-AP)

    IHC staining of rat spleen using 22225-1-AP (same clone as 22225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using MPO Polyclonal antibody (22225-1-AP)

    IHC staining of human spleen using 22225-1-AP (same clone as 22225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using MPO Polyclonal antibody (22225-1-AP)

    IHC staining of human spleen using 22225-1-AP (same clone as 22225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Polyclonal antibody (22225-1-AP)

    IHC staining of human liver using 22225-1-AP (same clone as 22225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 22225-1-AP (MPO antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using MPO Polyclonal antibody (22225-1-AP)

    IF Staining of human tonsillitis using 22225-1-AP (same clone as 22225-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 22225-1-AP (MPO antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using MPO Polyclonal antibody (22225-1-AP)

    IF Staining of human tonsillitis using 22225-1-AP (same clone as 22225-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MPO antibody (22225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using MPO Polyclonal antibody (22225-1-AP)

    IF Staining of mouse ovary using 22225-1-AP (same clone as 22225-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using MPO antibody (22225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using MPO Polyclonal antibody (22225-1-AP)

    IF Staining of human tonsillitis using 22225-1-AP (same clone as 22225-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 22225-1-AP (MPO antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22225-1-PBS in a different storage buffer formulation.

MPO Polyclonal antibody, PBS Only

MPO Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 22225-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

EC:1.11.2.2, myeloperoxidase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22225-1-PBS targets MPO in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17564

Product name: Recombinant human MPO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 606-657 aa of BC130476

Sequence: CGLPQPETVGQLGTVLRNLKLARKLMEQYGTPNNIDIWMGGVSEPLKRKGRV

相同性解析による交差性が予測される生物種
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC130476
Gene Symbol MPO
Gene ID (NCBI) 4353
RRIDAB_2879037
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP05164
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing. This antibody is specific to MPO.

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