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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using MPND Polyclonal antibody (24420-1-AP)

    WB analysis of SMMC-7721 using 24420-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 24420-1-AP (MPND Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MPND Polyclonal antibody (24420-1-AP)

    WB analysis of mouse liver using 24420-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24420-1-AP (MPND Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using MPND Polyclonal antibody (24420-1-AP)

    WB analysis of SMMC-7721 using 24420-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 24420-1-AP (MPND Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MPND Polyclonal antibody (24420-1-AP)

    WB analysis of HepG2 using 24420-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24420-1-AP (MPND Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MPND Polyclonal antibody

MPND Polyclonal Antibody for WB, ELISA
Cat No. 24420-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MPN domain containing, MPN domain containing protein, MPND

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSMMC-7721 cells, HepG2 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24420-1-AP targets MPND in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19531

Product name: Recombinant human MPND protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC032652

Sequence: MAAPEPLSPAGGAGEEAPEEDEDEAEAEDPERPNAGAGGGRSGGGGSSVSGGGGGGGAGAGGCGGPGGALTRRAVTLRVLLKDALLEPGAGVLSIYYLGKKFLGDLQPDGRIMWQETGQTFNSPSAWATHCKKLVNPAKKSGCGWASVKYKGQKLDKYKATWLRLHQLHTPATAADESPASEGEEEELLMEEEEEDVLAGVSAEDKSRRPLGKSPSEPAHPEATTPGKRVDSKIRVPVRYCMLGSRDLARNPHTLVEVTS

相同性解析による交差性が予測される生物種
Full Name MPN domain containing
Calculated molecular weight 471 aa, 51 kDa
Observed molecular weight 49-55 kDa
GenBank accession numberBC032652
Gene Symbol MPND
Gene ID (NCBI) 84954
RRIDAB_2879538
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N594
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MPND antibody 24420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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